Radioactively Labelled Phytic Acid from Maturing Seeds of Sinapis alba
نویسندگان
چکیده
Maturing seeds of Sinapis alba were incubated with D-[U-14C]glucose, sodium [l-14C]acetate or m>>0-[U-14C]inositol in order to prepare radioactively labelled phytic acid with high specific activity. Although each substrate was utilized for the biosynthesis of phytic acid, maximum incorporation of radioactivity into phytic acid was found with rayo-inositol. Radiochemical purity of the [U-14C]phytic acid preparations was confirmed by chromatographic techniques. Such preparations should be useful for the study o f interaction of phytic acid with metal ions and proteins and may serve as substrate in the assay of phytase.
منابع مشابه
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تاریخ انتشار 2013